Cryo-EM – Single Particle Analysis (SPA) in particular - has rapidly developed from a pioneering into a mainstream application that has caused a revolution in the field of structural biology at the atomic resolution. Significant resolution improvements on 3D protein structures and their interactive states have led to an increasing insight in protein machineries, sub-cellular interactions and mechanisms. This will eventually contribute to answer real biologically as well as medically relevant questions.
The results that have recently been obtained and published have triggered a large group of new users – mostly originating from the field of X-ray crystallography and NMR – to adopt cryo-EM as a viable, complementary technology. Now, finally, a continuum has been reached on all important aspects with regards to resolution and macromolecular scales which allows for the full deployment of the combination of these technologies.
We will discuss the future of structural biology based on the latest developments of the Thermo Fisher cryo-EM workflows and their components.